Phusion hot start ii manual "954"

I used Phusion Hot Start II DNA Polymerase (Biozym) and 1,5 mM MgCl2 concentrations for PCR. With reference to the manual provided for the kit, it does not involve a final extension step that PCR is not working for SDM. For more information I would read the manual from difficult construct I am using and the only one that worked well was OneTaq hot start polymerase by NEB. Thermo Scientific™ Phusion™ Hot Start II High-Fidelity DNA Polymerase Phusion Hot Start II High-Fidelity DNA Polymerase;100U (2U/uL) Thermo Phusion Hot Start II DNA Polymerase does not require a separate activation step in the PCR protocol as it is immediately reactivated at high temperatures. New Phusion Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion Hot Start II DNA Polymerase and 5X Phusion Green Buffers. Agilent also provides the Herculase Hotstart DNA Polymerase for applications that require high fidelity amplification over a broad target length range, and a specialized formulation of Stratagene high fidelity PfuTurbo DNA polymerase that includes hot start activity. Phusion Hot Start II DNA Polymerase does not require a separate activation step in the PCR protocol as it is immediately reactivated at high temperatures. New Phusion Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion Hot Start II DNA Polymerase and 5X Phusion Green Buffers. In hot-start PCR, Taq polymerase is inactive until heated. Hot-start PCR activation approaches allow users to minimize non-specific amplification while increasing target yield and specificity. How does our hot start technology work? Our JumpStart Taq DNA Polymerase is an antibody inactivated hot-start enzyme. Description Phusion Hot Start High-Fidelity DNA Polymerase is a highly accurate hot start DNA polymerase. It has now been upgraded to allow the use of even wider range of primers, including those with low melting temperatures. Use the NEB Tm Calculator to estimate an appropriate anne