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Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Capturing multiple two-dimensional images at different depths in a sample enables the ... download Light is a phenomenon that is explained with a model based on rays and wavefronts. The Olympus Microscopy Resource Center Microscopy Primer explores many of the aspects of visible light starting with an introduction to electromagnetic radiation. 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