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(A) Schematic representation of the expression cassettes for anti-CD3 scFv constructs. The locations of ribosome binding site (rbs), pelB leader sequence (pelB), either c-myc epitope followed by hexahistidine tag or hexahistidine tag alone (Tag) and stop codon (stop) are indicated. (B) The amino acid sequences of the V H –V L junctions in scFvs.