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Brunelle, Rachel Green, in Methods in Enzymology,. 2014 1 Theory. Polyacrylamide gel electrophoresis is useful for separating molecules by size and charge and there are many different systems depending on the sample and downstream applications. SDS-PAGE is a very useful tool to separate protein molecules by size. SDS is a detergent that denatures secondary and nondisulfide … The ancient Egyptians Laura Ingalls Wilders Fairy Poems Jason Carnes, Kenneth D. Stuart, in Methods in Enzymology,. 2007. 6.1 Overview. Helicase activity is not directly observed in either full‐round or precleaved editing assays, but it has been implicated in RNA editing in vivo (Panigrahi et al., 2003). Helicase KREH1 is a component of the editosome, and other helicases may also play a role in RNA editing. Because RNA helicases function by ... 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