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drug design or simply rational design, is the inventive process of finding new medications based on the knowledge of a biological target. The drug is most commonly an organic small molecule that activates or inhibits the function of a biomolecule such as a protein, which in turn results in a therapeutic benefit to the patient.
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Forensic Science An Introduction To Scientific And Investigative Te... download Quantitative Characterization Of Ligand Binding in ePub Receptor/
ligand binding affinity. The interaction of most ligands with their
binding sites can be characterized in terms of a
binding affinity. In general, high-affinity
ligand binding results from greater intermolecular force between the
ligand and its receptor while low-affinity
ligand binding involves less intermolecular force between the
ligand and its receptor.
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Ligand-binding assays are fundamental to characterizing biosimilars. During biosimilar development, it is necessary to demonstrate that the physicochemical properties, efficacy, and safety (e.g., immunogenicity) of the biosimilar are similar to those of the targeted branded biologic.
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ligand-binding assay is performed with reporter cells grown in DMEM without phenol red containing 5% charcoal-treated FCS. • To determine the RARα, RARβ, RARγ, and RXRβ agonistic/antagonistic potential of the ligands, seed equal amount (13,000 cells/well) of …
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